Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 45, Issue 4, Pages 321-329Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2009.08.003
Keywords
Lysozyme; Amyloid fibril; Redox composition
Funding
- National Science Council, Taiwan
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This study examined the effects of glutathione on the fibrillation of hen egg-white lysozyme. We found that the fibrillation of lysozyme was considerably reduced by GSH while no anti-aggregating activity was detected with only GSSG. SDS-PAGE results also revealed that the addition of GSH led to an early occurrence of prominent lysozyme hydrolysis. Moreover, GSH was effective in inhibiting lysozyme fibrillation when GSH was added within 6 days of incubation. We conclude that the attenuation of lysozyme fibrillation is strongly dependent upon the redox environment. Our data may contribute to decipher the molecular mechanism of amyloid fibrillation. (C) 2009 Elsevier B.V. All rights reserved.
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