Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 44, Issue 2, Pages 175-181Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2008.11.009
Keywords
Serum porcine albumin lactosylation; Maillard reaction; E. coli K88 adhesin biorecognition
Funding
- National Council of Science and Technology of Mexico
- CONACYT [P47998-Q]
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Escherichia coli (E. coli) that expresses galactose-reactive lectins, like K88 adhesin, causes high mortality among piglets. Carbohydrates that compete for adhesion could serve as an alternative for disease prevention. Porcine serum albumin (PSA) was modified by non-enzymatic glycation with lactose to produce PSA-Lac or PSA-GIc beta (1-4) Gal, as confirmed by reduction of available free amino groups, increased molecular mass and by Ricinus communis lectin recognition. E. coli K88 binds to PSA-Lac treatments containing three and four lactoses, respectively. In addition, PSA-Lac partially inhibited K88 strain adherence to mucins. These results suggest that neoglycoconjugates obtained by non-enzymatic glycation of proteins may serve in the prophylaxis of piglets' diarrhea. (C) 2008 Elsevier B.V. All rights reserved.
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