Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 42, Issue 2, Pages 133-137Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2007.10.007
Keywords
bovine serum albumin (BSA); intrinsic viscosity; conformers; Huggins' constant; Kraemer's constant; Mark-Houwink's constant
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Intrinsic viscosity of bovine serum albumin (BSA) at different pH values (2.7, 4.3, 7.4, 8 and 10) has been determined, as well as the Mark-Houwink constant and expansion factor. The traditional technique for data analysis using extrapolation to obtain intrinsic viscosity values shows an unusual behavior regarding concentration that can be observed in the values obtained for Huggins' and Kraemer's constants. (C) 2007 Elsevier B.V. All rights reserved.
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