Journal
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
Volume 53, Issue -, Pages 380-388Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.biocel.2014.06.004
Keywords
Long noncoding RNA; Sox2ot; Non-small cell lung cancer; EZH2; Proliferation
Categories
Funding
- Ministry of Science and Technology of the People's Republic of China [2011CB944104, 2010CB945101]
- National Natural Science Foundation [81172009, 81372168]
- Doctoral Fund of Ministry of Education of China [20110091120028]
- Fundamental Research Funds for the Central Universities [090314340001]
- Twelve-Five Plan the Major Program of Nanjing Medical Science and Technique Development Foundation
- Third Level Training Program of Young Talent Project of Nanjing Health, Nanjing Medical Science and Technology Development Project [ZDX12013]
- Young Professionals Foundation of Nanjing Chest Hospital
Ask authors/readers for more resources
Sox2 overlapping transcript (Sox2ot) is a long noncoding RNA (IncRNA), localized on human chromosome 3q26.33, which is frequently amplified in lung squamous cell carcinomas (SCCs). However, its roles in lung cancer remain under investigation. In this study, we found that Sox2ot was up-regulated over two folds in 53.01% of human primary lung cancers (44/83). The expression level of Sox2ot is significantly higher in SCCs than that in adenocarcinomas (ADCs) of the lung. Further study found high Sox2ot expression predicted poor survival in lung cancer patients (P=0.0053), implying Sox2ot is a novel prognostic factor. In two human lung cancer cell lines, HCC827 and SK-MES-1, knocking down Sox2ot inhibited cell proliferation by inducing G2/M arrest, with a concomitant decrease of cells in S phase. Reduced protein levels of Cyclin B1 and Cdc2 were also observed. Importantly, knocking down Sox2ot decreased EZH2 expression and reintroduction of EZH2 allowed Sox2ot knockdown cells progressed through G2/M phase, which correlates with the restoration of Cyclin B1 and Cdc2 expressions. Altogether, our data suggested that Sox2ot plays an important role in regulating lung cancer cell proliferation, and may represent a novel prognostic indicator for the disease. (C) 2014 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available