Journal
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
Volume 40, Issue 3, Pages 257-259Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijantimicag.2012.04.002
Keywords
beta-Lactamase; Insertion sequence; Genetic context; Transposon
Funding
- INSERM [U914]
- UMR Universite Paris-Sud (France)
- European Community (TEMPOtest-QC) [HEALTH-2009-241742]
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The genetic context of the gene coding for the novel variant of the class A beta-lactamase RTG-5 (CARB-14), exhibiting two amino acid substitutions compared with the closest RTG-3 enzyme, was analysed in a clinical isolate of Acinetobacter baumannii. This novel variant exhibited a narrow-spectrum profile, as observed for RTG-3. Sequence analysis of the region flanking the 5' end of bla(CARB-14) identified a novel insertion sequence ISAba21 belonging to the IS3 family. Downstream of bla(CARB-14), another insertion sequence belonging to the IS3 family named ISAba14 was identified. Analysis of the left-end extremity of ISAba21 and the right-end extremity of ISAba14 identified direct repeat sequences of 3 bp, likely being the signature of a transposition process. This is one of the few examples of a composite transposon made of different insertion sequences bracketing a gene coding for this novel carbenicillinase. (C) 2012 Elsevier B. V. and the International Society of Chemotherapy. All rights reserved.
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