4.7 Article

A novel and hybrid composite transposon at the origin of acquisition of blaRTG-5 in Acinetobacter baumannii

Journal

INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
Volume 40, Issue 3, Pages 257-259

Publisher

ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijantimicag.2012.04.002

Keywords

beta-Lactamase; Insertion sequence; Genetic context; Transposon

Funding

  1. INSERM [U914]
  2. UMR Universite Paris-Sud (France)
  3. European Community (TEMPOtest-QC) [HEALTH-2009-241742]

Ask authors/readers for more resources

The genetic context of the gene coding for the novel variant of the class A beta-lactamase RTG-5 (CARB-14), exhibiting two amino acid substitutions compared with the closest RTG-3 enzyme, was analysed in a clinical isolate of Acinetobacter baumannii. This novel variant exhibited a narrow-spectrum profile, as observed for RTG-3. Sequence analysis of the region flanking the 5' end of bla(CARB-14) identified a novel insertion sequence ISAba21 belonging to the IS3 family. Downstream of bla(CARB-14), another insertion sequence belonging to the IS3 family named ISAba14 was identified. Analysis of the left-end extremity of ISAba21 and the right-end extremity of ISAba14 identified direct repeat sequences of 3 bp, likely being the signature of a transposition process. This is one of the few examples of a composite transposon made of different insertion sequences bracketing a gene coding for this novel carbenicillinase. (C) 2012 Elsevier B. V. and the International Society of Chemotherapy. All rights reserved.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available