Journal
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
Volume 37, Issue 4, Pages 324-331Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijantimicag.2010.12.011
Keywords
Lipopeptide; Biosurfactant; Synergy; Biofilm; Eradication; Uropathogenic Escherichia coli
Funding
- Alberta Heritage Foundation for Medical Research through Universities Technology International
- Marcopolo S.p.A (Borgo San Dalmazzo, Cuneo, Italy)
- Natural Science Engineering Research Council of Canada
- Canadian Institutes of Health Research
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Biofilms are microcolonies of microbes adherent to biotic and abiotic surfaces, often responsible for chronic infections and medical device contamination. Escherichia coli is one of the prevalent pathogens involved in uropathogenic infections and contamination of catheters. Abiosurfactant produced by Bacillus licheniformis V9T14 was tested alone and in association with various antibiotics against a mature 24-h uropathogenic E. coli CFT073 biofilm. Biofilm was grown on polystyrene pegs of a Calgary Biofilm Device, providing a tool to evaluate the efficacy of antimicrobial agents. Antibiotics tested were ampicillin, cefazolin, ceftriaxone, ciprofloxacin, piperacillin, tobramycin and trimethoprim/sulfamethoxazole (19:1). Biosurfactant alone at the concentrations tested was not able to remove the adherent cells of the preformed biofilm. However, the difference between the effect of antibiotic alone and in combination with the biosurfactant was significant and exceeded 1 log(10) (90%) reduction in most cases. Results of this study indicate that V9T14 biosurfactant in association with antibiotics leads to a synergistic increase in the efficacy of antibiotics in biofilm killing, and in some combinations leads to total eradication of E. coli CFT073 biofilm. (c) 2011 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.
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