Journal
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
Volume 36, Issue 2, Pages 132-136Publisher
ELSEVIER
DOI: 10.1016/j.ijantimicag.2010.03.029
Keywords
Mycobacteria; Viability; Lantibiotics; AlamarBlue
Funding
- Institute of Technology Ireland
- Department of Agriculture
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The aim of this study was to use the microtitre alamarBlue assay to investigate and compare the antimycobacterial potential of the lantibiotics nisin and lacticin 3147 against a representative cohort of clinically significant mycobacteria, i.e. Mycobacterium tuberculosis H37Ra, Mycobacterium avium subsp. paratuberculosis (MAP) ATCC 19698 and Mycobacterium kansasii CIT11/06. Lacticin 3147 displayed potent activity against all strains of mycobacteria, with MIC90 values (lowest concentration of lantibiotic that prevented growth of >90% of the bacterial population) of 60 mg/L and 15 mg/L for M. kansasii and MAP, respectively. Lacticin 3147 was particularly effective against M. tuberculosis H37Ra, with a MIC90 value of 7.5 mg/L. Nisin, although inhibitory, was generally less potent against all strains of mycobacteria, with MIC90 values of 60 mg/L for M. kansasii and >60 mg/L for MAP and M. tuberculosis H37Ra. Thus, lacticin 3147 is a potent antimycobacterial peptide that shows superior activity compared with nisin at physiological pH. (C) 2010 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.
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