Journal
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
Volume 35, Issue 3, Pages 255-260Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijantimicag.2009.11.017
Keywords
L. acidophilus; Probiotic; Antimicrobial peptide; Pathogenic microorganisms
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In the last two decades, antimicrobial peptides (AMPs) have been gaining attention as antimicrobial alternatives to chemical food preservatives and commonly used antibiotics. Lactobacillus acidophilus n.v. Er 317/402 strain Narine produces a small AMP with a molecular weight of 1.1 kDa, designated acidocin LCHV. In this study, the AMP was extremely heat stable (90 min at 130 degrees C), was active over a wide pH range and was found to be sensitive to proteolytic enzymes (trypsin, pepsin and proteinase K). Acidocin LCHV has a broad spectrum of activity both against Gram-positive and Gram-negative pathogens, including several that are classified as Especially Dangerous Infections by the World Health Organization as well as meticillin-resistant Staphylococcus aureus (MRSA) and Clostridium difficile. Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF/MS) was used to determine the molecular mass and sequence of the purified peptide. Complete killing with immediate impact on cells was observed within a very short period of time (10 min). (C) 2009 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.
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