Journal
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
Volume 34, Issue 6, Pages 602-604Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijantimicag.2009.06.029
Keywords
Overexpression; Efflux pumps; MDR phenotype
Funding
- [EU-FSE/FEDER-POCI/SAU-MMO/59370/2004]
- [EU-FSE/FEDER-PTDC/BIA-MIC/71280/2006]
- [SFRH/BPD/34578/2007]
- [SFRH/BD/19445/2004]
- [SFRH/BD/24931/2005]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/24931/2005] Funding Source: FCT
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In this study, we aimed to answer the following question: 'How does a bacterium become so resistant to a given antibiotic even though the levels of antibiotic to which it has become resistant remained constant in the patient?' Escherichia coli AG100 strain induced to high-level resistance due to overexpression of an AcrAB efflux pump was serially cultured in 10 mg/L tetracycline for 60 passages. Between each passage it became increasingly resistant to tetracycline, beta-lactams and quinolones with concomitant restoration of wild-type AcrAB activity. Because the multidrug-resistant phenotype could not be reversed with transfer to drug-free medium or with efflux pump inhibitors, it may have resulted from activation of a 'mutator gene' system that reduced the 'energy consumption' associated with an overexpressed efflux pump system. (C) 2009 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.
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