Journal
INTERNATIONAL JOURNAL FOR PARASITOLOGY
Volume 42, Issue 1, Pages 63-70Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.ijpara.2011.10.006
Keywords
Venom allergen-like protein; Bursaphelenchus xylophilus; Parasitism; Relative transcript level; Immunohistochemistry; RNA interference; Migration assay
Categories
Funding
- Korea Forest Service [500-20060168]
Ask authors/readers for more resources
The pinewood nematode, Bursaphelenchus xylophilus, is a destructive pest in several countries including Japan, China and Korea. Of three genes encoding the venom allergen-like protein in B. xylophilus, Bxvap-1 showed the highest transcript levels at the pine-grown propagative stage. In addition, western blot and immunohistochemical analyses using anti-BxVap-1 polyclonal antibody verified a specific increase in BxVap-1 expression levels at the pine-grown propagative stage. Using immunohistochemistry, BxVap-1 was detected around the putative oesophageal glands and metacarpus, suggesting that BxVap-1 is secreted into the host pine tree and is involved in the parasitic mechanism. To explain the parasitic role of BxVap-1, we measured the migration rate inside pine seedlings of B. xylophilus either with or without Bxvap-1 knockdown by RNA interference. Bxvap-1 knockdown resulted in a significantly lower migration rate in the >6 cm region compared with the control B. xylophilus. These results suggest that BxVap-1 is involved in B. xylophilus migration, perhaps by suppressing the pine tree defence mechanism. (C) 2011 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available