Journal
INTERNATIONAL JOURNAL FOR PARASITOLOGY
Volume 39, Issue 14, Pages 1551-1559Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.ijpara.2009.06.006
Keywords
Microarray; Schistosoma japonicum; Gene expression; Insulin
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Funding
- University of Queensland
- National Health and Medical Research Council of Australia
- Wellcome Trust (UK)
- Sandler Foundation (USA)
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Microarray analysis was used to investigate differential gene regulation in adult male and female Schistosoma japonicum cultured in the presence or absence of insulin in vitro. A total of 1,101 genes were up- or down-regulated in response to insulin, the majority of differential expression occurring 24 h after the addition of insulin to the cultures. Genes differentially expressed in male or female worms were predominantly involved in growth and development, with significant sex-specific differences in transcriptional profiles evident. Insulin appeared to promote protein synthesis and control protein degradation more prominently in male parasites. The study also indicated that insulin plays a more pronounced role in the uptake of glucose in unpaired female parasites, as reflected in the increased stimulation of gene expression of the phosphatidylinositol 3-kinase sub-pathway of insulin signalling. Insulin may also impact on the sexual differentiation and fecundity of female schistosomes by activation of the mitogenic-activated protein kinase sub-pathway. (C) 2009 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.
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