Journal
INTERNATIONAL JOURNAL FOR PARASITOLOGY
Volume 38, Issue 11, Pages 1297-1304Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.ijpara.2008.04.012
Keywords
Giardia; diplomonads; nucleolus; nucleolar organising region; ribonucleoproteins; rRNA processing
Categories
Funding
- CONACyT-Mexico [62321, 49724]
- DGAPA-UNAM PAPIIT [IN215106]
- Swiss National Science Foundation [3100A0-112327]
Ask authors/readers for more resources
Giardia duodenalis has been described as 'anucleolated'. In this work we analysed the subcellular distribution of several nucleolar markers in Giardia nuclei using silver and immunostaining techniques for electron and confocal laser microscopy as well as expression of epitope-tagged proteins in transgenic trophozoites. We identified anteronuclear fibrogranular structures corresponding to nucleolar organising regions with recruited ribonucleoprotein complexes, rRNA and epitope-tagged fibrillarin and rRNA-pseudouridine synthase (CBF5). Recombinant fibrillarin and CBF5 were targeted to this subcompartment. This study demonstrates the presence of nucleoli in G. duodenalis and provides a model to analyse minimal requirements for nucleolar assembly and maintenance in eukaryotic cells. (C) 2008 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available