Journal
INTERNATIONAL IMMUNOPHARMACOLOGY
Volume 23, Issue 1, Pages 294-303Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.intimp.2014.09.005
Keywords
Ulcerative colitis; Baicalin; TLR4; NF-kappa B; Pathway
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Funding
- Natural Science Foundation of China [81403121, 81303275]
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Background: Baicalin holds a protective effect on inflammatory responses in several diseases. However, its molecular mechanism of anti-inflammatory activity on ulcerative colitis (UC) remains unknown. The present study was conducted to verify whether the anti-inflammation effect of baicalin on experimental colitis is via inhibiting TLR4/NF-kappa B pathway activation. Methods: The inflammatory response in RAW264.7 cells was induced by LPS and in rats by intrarectal administration of TNBS. Western blot analysis was carried out to examine toll-like receptor 4 (TLR4), NF-kappa B, p-NF-kappa B p65, I kappa B and p-I kappa B protein expressions in cells. Furthermore, intercellular adhesion molecule-1 (ICAM-1), monocyte chemoattractant protein-1 (MCP-1), cyclo-oxygenase-2 (Cox-2), tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-1 beta and IL-6 levels in cell supernatant and rat serum were detected by appropriate kits. An immunohistochemical assay was applied to examine TNF-alpha and IL-1 beta protein expression in colon tissues and TLR4 and p-NF-kappa B p65 protein expressions in RAW264.7 cells. Results: Baicalin ameliorates the considered inflammatory symptoms of induced colitis. It could also down-regulate pro-inflammatory mediators in the colon mucosa. The decline in the production of pro-inflammatory cytokines was correlated with the decrease in mucosal TLR4 protein expression. The expression of p-NF-kappa B p65 protein was significantly decreased, which correlated with a similar decrease in p-I kappa B protein. Consistent with the in vivo results, baicalin blocked LPS-stimulated nuclear translocation of p-NF-kappa B p65 in mouse macrophage RAW264.7 cells. Conclusions: The present study indicates for the first time that the mechanism for baicalin on abrogating experimental colitis was targeted inhibition of the TLR4/NF-kappa B pathway activation. (C) 2014 Elsevier B.V. All rights reserved.
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