Journal
INTERNATIONAL IMMUNOPHARMACOLOGY
Volume 23, Issue 2, Pages 617-623Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.intimp.2014.10.014
Keywords
Resveratrol; Inflammation; High mobility group box protein 1; Nuclear factor kappa B; Cytokine; Receptor for advanced glycation end products
Categories
Funding
- Ministry of Education, Culture, Sports, Science and Technology of Japan [23602012]
- Promotion and Mutual Aid Corporation for Private Schools, Japan [26460239]
- Grants-in-Aid for Scientific Research [23602012] Funding Source: KAKEN
Ask authors/readers for more resources
Resveratrol is a polyphenol abundantly found in red grape skin and is effective against antiaging and anti-inflammation associated with immune responses. In this study, we have investigated the effect of resveratrol on skin lesion, high mobility group box (HMGB)1 and inflammation pathway in an atopic dermatitis (AD) mouse model. AD-like lesion was induced by the application of house dust mite extract to the dorsal skin of NC/Nga mouse. After AD induction, resveratrol (20 mg/kg, p.o.) was administered daily for 2 weeks. We evaluated dermatitis severity, histopathological changes, serum levels of T helper (Th) cytokines (interferon (IFN)gamma, interleukin (IL)-4) and changes in protein expression by Western blotting for HMGB1, receptor for advanced glycation end products (RAGE), toll like receptor (TLR)4, nuclear factor (NF)kappa B, phosphatidylinositide 3-kinase (PI3K), extracellular signal-regulated kinase (ERK)1/2, cyclooxygenase (COX)2, tumor necrosis factor (TNF)alpha, IL-1 beta IL-2R alpha and other inflammatory markers in the skin of AD mice. Treatment of resveratrol inhibited the development of the AD-like skin lesions. Histological analysis showed that resveratrol inhibited hypertrophy, intracellular edema, mast cells and infiltration of inflammatory cells. Furthermore, resveratrol treatment down-regulated HMGB1, RAGE, p-NF kappa B, p-PI3K, p-ERK1/2, COX2, TNF alpha, IL-1 beta, IL-2R alpha, IFN gamma and IL-4. Considering all these findings together, the HMGB1 pathway might be a potential therapeutic target in skin inflammation, and resveratrol treatment could have beneficial effects on AD by modulating the HMGB1 protein expression. (C) 2014 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available