Journal
INTERNATIONAL IMMUNOPHARMACOLOGY
Volume 15, Issue 2, Pages 246-253Publisher
ELSEVIER
DOI: 10.1016/j.intimp.2012.12.008
Keywords
Acute lung injury; Mesenchymal stem cells; Transplantation; Endotoxemia; Inflammation
Categories
Funding
- Special Funds for Major State Basic Research Projects [2012CB518104]
- Open Fund of State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University [2009ZZ008]
- National Natural Sciences Foundation of China [31271242]
- Medical Research Funding of PLA [ASW11J008]
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Previous studies have demonstrated that acute lung injury (ALI) is associated with significant mortality and so far no effective pharmacotherapy is shown to reverse the natural progression. This study aims to investigate whether the transplantation of bone marrow derived mesenchymal stem cells (MSCs) might restore damaged pulmonary function and tissue structure in ALI. By using a sublethal chest impact injury plus endotoxemia model, MSCs were intravenously transplanted into the injured rats 2 h after trauma. The blood samples were obtained for determination of blood gas. Bronchoalveolar lavage fluid (BALF) was collected for analysis of inflammatory cell count and cytokine (TNF-alpha, IL-1 beta, IL-6 and IL-10) levels. The left lower lobes of the lungs, nearby the impact zone, were collected for SRY gene analysis and histological examination and scoring. After engraftment of MSCs, the number of inflammatory cells in BALE was decreased. Meanwhile, the secretions of pro-inflammatory TNF-alpha and IL-6 were alleviated, while the secretion of anti-inflammatory IL-10 was elevated. Engraftment of MSCs improved the pulmonary gas exchanges, alleviated the lung injury, and reduced the rats' mortality. These results suggested that the MSCs based measures might be a promising strategy in trauma and endotoxemia induced ALI treatment. (C) 2012 Elsevier B.V. All rights reserved.
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