Journal
INTERNATIONAL IMMUNOPHARMACOLOGY
Volume 11, Issue 11, Pages 1946-1953Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.intimp.2011.06.006
Keywords
SEP; Myelosuppression; Immunosuppression; PI3K/Akt
Categories
Funding
- National Science and Technology Major Project Foundation of China [2009ZX09103-655]
- Ministry of Education of China and State Administration of Foreign Expert Affairs of China [111-2-07]
- Qing Lan Project from Jiangsu Province
- National Innovation Experiment Program for University Students
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To assess the chemoprotective properties of a polysaccharide from Strongylocentrotus nudus eggs (SEP), myelosuppressed and immunosuppressed mouse models were generated by administration of cyclophosphamide (Cy) and then treated with SEP. SEP (16 mg/kg/d) remarkably increased spleen and thymus indices, activated the proliferation of leukocytes and erythrocytes and platelets from peripheral blood, and exhibited co-mitogenic activity on ConA- or LPS-stimulated splenocytes in a dose-dependent manner. An increased percentage of CD34(+) cells in bone marrow of Cy-treated mice was also observed. Furthermore, SEP elevated CD4(+) T lymphocyte counts as well as the CD4/CD8 ratio dose-dependently, and it increased interleukin-2 (IL-2), IgA, IgM, and IgG levels in the sera of Cy-treated mice. Pre-incubation with TLR2 and TLR4 blocking antibodies inhibited splenocyte proliferation and its IL-2 secretion. Finally. SEP significantly induced Akt phosphorylation in splenocytes from Cy-treated mice, suggesting that chemoprotection by SEP was mediated through the PI3K/Akt signaling pathway. These findings indicate that SEP plays an important role in the protection against myelosuppression and immunosuppression in Cy-treated mice and could be a potential immunomodulatory agent. (C) 2011 Elsevier B.V. All rights reserved.
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