The effect of taurine on the toll-like receptors/nuclear factor kappa B (TLRs/NF-κB) signaling pathway in Streptococcus uberis-induced mastitis in rats

To investigate whether taurine ameliorates mammary damage in a rat model of S. uberis mastitis by suppressing inflammation related to the toll-like receptors/nuclear factor kappa B (TIRs/NF-kappa B) signaling pathway. Starting on gestation day 14 and continuing until parturition, 100 mg/kg of taurine (group TS) or an equal volume of physiological saline (group CS) was administered daily to rats. Seventy-two hours after parturition, rats were infused with 100 cfu of S. uberis into each of 2 mammary glands. The resultant inflammation, evidenced by swelling, degeneration of secretory epithelium, increased tissue loss and neutrophil (PMN) infiltration was observed. Pretreatment with taurine attenuated inflammatory changes and significantly decreased mRNA expression of TLR-2 (8 h post S. uberis-injection, PI), NF-kappa B p65 (16 h and 24 h PI), and NF-kappa B DNA binding activity (16 h PI). Tumor necrosis factor (TNF)-alpha and inducible nitric oxide synthase (iNOS) levels were also decreased. Significant differences (P < 0.05) were present at 24 h and 48 h PI for TNF-alpha and at 16 h PI for iNOS. TLR-4 mRNA expression was increased by taurine administration and significant differences were observed at 8 h, 16 h and 24 h PI. These results suggest that the in vivo relationship of immunomodulatory reagents with TLRs is complex. Taurine may modulate inflammatory injury induced by S. uberis in mammary glands though TLR-2 and TLR-4. Suppression of inflammation may be related to TLRs/NF-kappa B and may be one mechanism of taurine action in controlling S. uberis mastitis. (C) 2011 Elsevier B.V. All rights reserved.