Journal
INTERNATIONAL IMMUNOPHARMACOLOGY
Volume 11, Issue 12, Pages 1984-1990Publisher
ELSEVIER
DOI: 10.1016/j.intimp.2011.08.008
Keywords
Berberine; Interleukin-2; Interleukin-4; Interleukin-10; Tumor necrosis factor alpha
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Funding
- National Science Council, Taipei, Taiwan, ROC [NSC98-2313-B-005-035-MY3]
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Berberine is a natural isoquinoline alkaloid. This study investigated the effects of berberine on cytokine gene expression in mouse primary splenocytes in the absence or presence of lipopolysaccharide (U'S) using 4 different experimental models in vitro. The relative expression of the following cytokine genes was determined using a real-time quantitative polymerase chain reaction assay: pro-inflammatory tumor necrosis factor (TINIF)-alpha, anti-inflammatory interleukin (IL)-10. T-helper type 1 (Th1) (IL-2), and Th2 (IL-4) cytokines. The results showed that berberine down-regulated ratios of the relative Th1 (IL-2)/Th2 (IL-4) cytokines expression fold in mouse primary splenocytes in the absence or presence of LPS in a preventive manner. This study suggests that berberine may possess anti-inflammatory potential by shifting the Th1/Th2 balance toward Th2 polarization. (C) 2011 Elsevier B.V. All rights reserved.
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