Journal
INTERNATIONAL IMMUNOPHARMACOLOGY
Volume 9, Issue 4, Pages 508-513Publisher
ELSEVIER
DOI: 10.1016/j.intimp.2009.01.006
Keywords
Cucurbitacin D; Trichosanthes kirilowii; Hepatocellular carcinoma; Apoptosis; Caspase-3; JNK
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Funding
- Ministry of Education, Science, Sports and Cultureof Japan [205921329]
- Advanced Research from University of Occupational and Environmental Health, Japan
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The aim of the present study is to examine the effects of the anti-tumor component isolated from Trichosanthes kirilowii on human hepatocellular carcinoma cells. Using Sephadex G-25 Column Chromatography, Sep-Pak Plus C18 cartridge and high-performance liquid Chromatography (HPLC), we isolated the active component from trichosanthes extract. By fast atom bombardment mass spectrometric analysis, the molecular mass of the active fraction was determined, the active components identified, and their mechanisms of action were analyzed by cell growth assay, cell cycle analysis, TUNEL staining and Western blot analysis. We found that the anti-tumor components isolated from the extract of trichosanthes (EOT) are cucurbitacin D and dihydrocucurbitacin D, and suggest that cucurbitacin D induces apoptosis through caspase-3 and phosphorylation of JNK in hepatocellular carcinoma cells. These results suggest that cucurbitacin D isolated from Trichosanthes kirilowii could be a valuable candidate for anti-tumor drug. (C) 2009 Elsevier B.V. All rights reserved.
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