Dynamics of T-cell IFN-γ and miR-29a expression during active pulmonary tuberculosis

IFN-gamma is crucial for protection against Mycobacterium tuberculosis. miR-29 was recently shown to non-redundantly inhibit IFN-gamma. Here, we investigated IFN-gamma and miR-29a expression dynamics of CD4(+) T cells from patients during active tuberculosis (TB) (n = 32) and in household contacts who were latently M. tuberculosis infected (n = 19) from Ghana. Whereas M. tuberculosis-specific IFN-gamma expression was similar during TB chemotherapy, superantigen stimulation indicated generally impaired IFN-gamma expression in TB patients. No interdependency between miR-29a and IFN-gamma expression of T cells was observed. However, miR-29a was differentially expressed in T cells during chemotherapy. We concluded that differential miR-29a expression in active TB was not causative for impaired IFN-gamma expression.