Journal
INTERNATIONAL IMMUNOLOGY
Volume 20, Issue 9, Pages 1211-1218Publisher
OXFORD UNIV PRESS
DOI: 10.1093/intimm/dxn079
Keywords
hematopoietic stem cells; manipulation of immune system; NUP98-HOXB4
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Funding
- Biomedical Research Council [06/1/22/19/471, 06/1/22/19/469]
- Singapore Immunology Network [SiGN-06-004]
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Extensive amplification of hematopoietic stem cells (HSCs) and their multipotent primitive progenitors (MPPs) in culture would greatly benefit not only clinical transplantation but also provide a potential tool to manipulate all cellular lineages derived from these cells for gene therapy and experimental purposes. Here, we demonstrate that mouse bone marrow cultures containing cells engineered to over-express NUP98-HOXB4 fusion protein support self-renewal of physiologically normal HSC and MPP for several weeks leading practically to their unlimited expansion. This allows time consuming and cumulative in vitro experimental manipulations without sacrificing their ability to differentiate in vivo or in vitro to any hematopoietic lineage.
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