Journal
INTERNATIONAL ENDODONTIC JOURNAL
Volume 43, Issue 5, Pages 404-412Publisher
WILEY
DOI: 10.1111/j.1365-2591.2010.01693.x
Keywords
differentiation; human dental papilla cells; mineralization; Wnt5a
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Funding
- Sichuan Province science fund for distinguished young scholars [08ZQ026-032]
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P>Aim To investigate the role of Wnt5a in the process of differentiation of human dental papilla cells (HDPCs). Methodology Recombinant adenovirus encoding full-length Wnt5a cDNA was constructed to investigate the biological role of Wnt5a on the differentiation of HDPCs. The effect of Wnt5a on HDPCs differentiation was determined by ALP activity assay, ALP staining and mineral induction assay. Mineralization-related gene expressions were assessed by RT-PCR. Results Immunostaining revealed Wnt5a expression in the odontoblast layer and dental papilla tissue. Over-expression of Wnt5a by transfecting HDPCs with an Wnt5a-carrying construct increased ALPase activity and the formation of mineralized nodules of HDPCs. RT-PCR analysis showed that the expressions of mineralization-related genes, such as bone sialoprotein, collagen type I, osteonectin, osteopontin (OCN), dentine matrix protein-1 were up-regulated by Wnt5a. Conclusions Wnt5a promoted differentiation of HDPCs.
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