Journal
INTERNATIONAL DAIRY JOURNAL
Volume 18, Issue 9, Pages 923-927Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.idairyj.2008.02.001
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This study demonstrates the optimization of AS-48 production by strain Enterococcus faecalis A-48-32 using a whey-derived substrate, Esprion-300 (E-300). To optimise AS-48 production, various factors such as E-300 concentration, glucose, pH and inoculum size were adjusted. The following optimal conditions were established: a growth medium comprising 5% E-300 and 1% glucose, inoculated with 8% producer strain and incubated at 28 degrees C with a controlled pH of 6.55. Under these conditions a maximum activity of 360 AU mL(-1) was attained after 18 h and remained relatively constant for 24 h. The critical factors for optimal AS-48 production in this medium were the pH stabilization at 6.55, in addition to a 1% glucose concentration. (C) 2008 Elsevier Ltd. All rights reserved.
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