Journal
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
Volume 86, Issue -, Pages 34-41Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.ibiod.2013.03.024
Keywords
Biofilm control; Disinfection; Drug resistance; Escherichia colt; Phenyl isothfocyanate; Staphylococcus aureus
Funding
- Operational Programme for Competitiveness Factors - COMPETE
- FCT - Portuguese Foundation for Science and Technology [PTDC/EBB-EB1/105085/2008, PTDC/DTP-SAP/1078/2012]
- PhD grant [SFRH/BD/84393/2012, SFRH/BD/63398/2009]
- Fundação para a Ciência e a Tecnologia [PTDC/DTP-SAP/1078/2012] Funding Source: FCT
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The purpose of the present study was to assess the antibacterial activity of phenyl isothiocyanate (PITC), a synthetic isothiocyanate, on biofilms of Escherichia coli and Staphylococcus aureus. The effects of PITC on bacterial free energy of adhesion and motility were also investigated. Biofilm formation in 96-well polystyrene microtiter plates was quantified by crystal violet staining and the metabolic activity of those biofilms was assessed with alamar blue. The viability and culturability of the biofilm bacteria after exposure to PITC were determined. The highest removal and metabolic activity reduction of biofilms with PITC was around 90% for both bacteria. Treatment with PITC enabled 4.5 and 4.0 log(10) reductions of the number of viable cells for E. coli and S. aureus, respectively; and no colony forming units (CFUs) were detected. PITC also affected the adhesion process and motility of bacteria, greatly preventing biofilm formation. In conclusion, PITC enabled both biofilm prevention and control, promoting high biofilm removal and inactivation activities, suggesting that this compound is a promising disinfectant. (C) 2013 Elsevier Ltd. All rights reserved.
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