Journal
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
Volume 78, Issue -, Pages 1-6Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.ibiod.2012.12.006
Keywords
Autofluorescence; Cell staining; Chlorella sorokiniana; Oxidative stress; TiO2 photocatalysis; Viability assay
Funding
- R&D projects for standards development for SMEs (Small and Medium Enterprises)
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We examined Chlorella cells exposed to several kinds of oxidative stress, namely photocatalysis, UV light, heat, and H2O2. After these oxidative treatments, the Chlorella cells were stained with neutral red, methylene blue, eosin Y, or fluorescein diacetate (FDA). The results of FDA staining were generally the most consistent with those of colony-forming unit assays. In addition, chlorophyll autofluorescence was responsive to photocatalytic oxidation and may be useful as a simple, rapidly obtained index of the viability of Chlorella cells exposed to photocatalysis. We also discussed differences in the stainability of cells in terms of external and internal cellular damage. Photocatalytic oxidation resulted in the greatest degree of cellular damage, including lipid peroxidation and cell deformation. (c) 2012 Published by Elsevier Ltd.
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