Journal
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
Volume 65, Issue 5, Pages 696-702Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.ibiod.2011.02.009
Keywords
Bauhinia monandra; Root lectin; Antifungal activity; Termiticidal activity
Funding
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
- Fundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco (FACEPE)
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This work describes the purification in milligram quantities of a lectin from Bauhinia monandra secondary roots (BmoRoL) and its antifungal and termiticidal activities. The BmoRoL (6.2 mg) was isolated through ammonium sulfate fractionation and affinity chromatography on guar gel. Native lectin was resolved as a single band on polyacrylamide gel electrophoresis for basic proteins. Under denaturing and reducing conditions it appeared as a unique glycosylated polypeptide of 26 kDa. The highest agglutination activity of BmoRoL was found with glutaraldehyde-treated rabbit erythrocytes. BmoRoL showed antifungal activity against phytopathogenic species of Fusarium and was more active on Fusarium solani. The lectin also showed termiticidal activity on Nasutitermes corniger workers and soldiers with LC50 of 0.09 and 0.395 mg ml(-1) for 12 days. In conclusion, BmoRoL is a new antifungal and termiticidal lectin that can be purified in milligram quantities and has potential biotechnological application for control of agricultural pests. (C) 2011 Elsevier Ltd. All rights reserved.
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