Journal
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY
Volume 155, Issue 2, Pages 119-128Publisher
KARGER
DOI: 10.1159/000318854
Keywords
Airway remodeling; Epithelial mesenchymal transition; TGF-beta 1; TNF-alpha
Categories
Funding
- Ministry of Education, Science, Sports, Culture, and Technology of Japan
- Ministry of Health, Labour, and Welfare of Japan
Ask authors/readers for more resources
Background: Airway remodeling is an important feature of chronic airway disease, but the mechanisms involved remain unclear. Recently, epithelial mesenchymal transition (EMT) was reported to be associated with tissue fibrosis. TGF-beta 1, which is a potent inducer of EMT, is thought to be related to the pathogenesis of airway remodeling. We investigated whether TGF-beta 1 and/or TNF-alpha induce EMT in bronchial epithelial cells. Methods: Cultured BEAS-2B cells and primary normal human bronchial epithelial cells (NHBE) were treated with TGF-beta 1 and/ or TNF-alpha. Morphological changes and the expression of EMT-related markers were evaluated by immunocytochemical staining. Expressions of EMT-related markers, extracellular matrix (ECM) components (collagen type I and versican), and TGF-beta receptors I, II, and III were analyzed by quantitative RT-PCR. Migration was evaluated using the Boyden chamber technique. Results: The TGF-beta 1-induced EMT in BEAS-2B cells was demonstrated on the basis of morphological changes and the downregulation of E-cadherin. Costimulation with TNF-alpha enhanced the TGF-beta 1-induced morphological changes and increased vimentin expression. Treatment with TGF-beta 1 increased the expression of collagen type I and versican. EMT induced with TGF-beta 1 plus TNF-alpha promoted cell migration. Stimulation of NHBE with TGF-beta 1 led to EMT. Conclusion: TGF-beta 1 induced EMT in BEAS-2B cells, and costimulation with TNF-alpha enhanced the EMT. As a result of the EMT process, BEAS-2B cells acquired functions of mesenchymal cells. In addition, TGF-beta 1 treatment induced EMT in NHBE as shown by changes in EMT-related markers. Bronchial epithelial cells might contribute to airway remodeling through EMT. Copyright (c) 2010 S. Karger AG, Basel
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available