Journal
INTEGRATIVE BIOLOGY
Volume 3, Issue 12, Pages 1188-1196Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c1ib00018g
Keywords
-
Categories
Funding
- Arizona Biomedical Research Commission
- Arizona Department of Health Services
- Michael J. Fox Foundation
- Office of Naval Research [N00014-07-1-0457]
- National Institute on Aging [P30 AG19610]
- Arizona Department of Health Services [211002]
- Arizona Biomedical Research Commission [4001, 0011, 05-901, 1001]
- Prescott Family Initiative of the Michael J. Fox Foundation
- NATIONAL INSTITUTE ON AGING [P30AG019610] Funding Source: NIH RePORTER
Ask authors/readers for more resources
Protein misfolding and aggregation is a critically important feature in many devastating neurodegenerative diseases, therefore characterization of the CSF concentration profiles of selected key forms and morphologies of proteins involved in these diseases, including beta-amyloid (A beta) and alpha-synuclein (a-syn), can be an effective diagnostic assay for these diseases. CSF levels of tau and A beta have been shown to have great promise as biomarkers for Alzheimer's disease. However since the onset and progression of many neurodegenerative diseases have been strongly correlated with the presence of soluble oligomeric aggregates of proteins including various A beta and a-syn aggregate species, specific detection and quantification of levels of each of these different toxic protein species in CSF may provide a simple and accurate means to presymptomatically diagnose and distinguish between these diseases. Here we show that the presence of different protein morphologies in human CSF samples can be readily detected using highly selective morphology specific reagents in conjunction with a sensitive electronic biosensor. We further show that these morphology specific reagents can readily distinguish between post-mortem CSF samples from AD, PD and cognitively normal sources. These studies suggest that detection of specific oligomeric aggregate species holds great promise as sensitive biomarkers for neurodegenerative disease.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available