Journal
INTEGRATIVE BIOLOGY
Volume 3, Issue 2, Pages 134-141Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c0ib00018c
Keywords
-
Categories
Funding
- Nano Systems Institute-National Core Research Center (NSI-NCRC)
- World Class University (WCU) Program [R31-2008-000-10083-0]
- Korea Research Foundation (MOEHRD) [KRF-J03003]
- Ministry of Education, Science and Technology, Korea [R01-2007-000-20441-0, 2010-0008225]
- Ministry of Health Welfare [A080143]
Ask authors/readers for more resources
In vivo, renal tubular epithelial cells are exposed to luminal fluid shear stress (FSS) and a transepithelial osmotic gradient. In this study, we used a simple collecting-duct-on-a-chip to investigate the role of an altered luminal microenvironment in the translocation of aquaporin-2 (AQP2) and the reorganization of actin cytoskeleton (F-actin) in primary cultured inner medullary collecting duct (IMCD) cells of rat kidney. Immunocytochemistry demonstrated that 3 h of exposure to luminal FSS at 1 dyn cm(-2) was sufficient to induce depolymerization of F-actin in those cells. We observed full actin depolymerization after 5 h exposure and substantial re-polymerization within 2 h of removing the luminal FSS, suggesting that the process is reversible and the fluidic environment regulates the reorganization of intracellular F-actin. We demonstrate that several factors (i.e., luminal FSS, hormonal stimulation, transepithelial osmotic gradient) collectively exert a profound effect on the AQP2 trafficking in the collecting ducts, which is associated with actin cytoskeletal reorganization.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available