Journal
INTEGRATIVE BIOLOGY
Volume 3, Issue 1, Pages 48-56Publisher
OXFORD UNIV PRESS
DOI: 10.1039/c0ib00067a
Keywords
-
Categories
Funding
- NIH [RR199652, EB005753]
- NATIONAL CENTER FOR RESEARCH RESOURCES [R01RR019652] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [R21EB005753] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM090194] Funding Source: NIH RePORTER
Ask authors/readers for more resources
Microsystems are increasingly used in the manipulation, patterning and sorting of cells. Critical to the widespread adoption of these new technologies is development of an understanding of their impact on cellular physiology. Here we show the integration of a cell-based sensor, a microfabricated electrical screening platform, and quantitative imaging to enable the first large-scale physiological screens of the impact of microsystems on cells. To perform physiological screening, we developed a cell-based sensor that reports on stress-mediated transcription (via Heat Shock Factor 1 induced expression of GFP). This cell-based sensor was quantitatively characterized using automated imaging. The integration of this quantitative physiological sensor with a microfabricated system enabled the execution of multiplexed screens across electric field strength, frequency, and application duration. Voltage sweeps indicate increasing physiological stress with increasing voltage due to Joule heating, while frequency sweeps indicate increased stress at lower frequencies (<500 kHz) compared with higher frequencies (> 1 MHz) due to generation of reactive species at lower frequencies. Combined voltage and frequency sweeps enable the generation of complex maps of physiological state.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available