Journal
MEDICAL ONCOLOGY
Volume 32, Issue 2, Pages -Publisher
HUMANA PRESS INC
DOI: 10.1007/s12032-014-0444-3
Keywords
SHARPIN; Prostate cancer; Tumorigenesis; NF-kappa B/ERK/Akt pathway; Apoptosis
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Funding
- National Natural Science Foundation of China [81272807, 81472382]
- National Natural Science Foundation of China for Young Scientists Grant [81101947]
- Science and Technology Development Program of Guangdong Province [2013B021800107]
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SHARPIN emerges higher expression in prostate cancerous tissues than in benign prostate hyperplasia by means of immunohistochemistry in our previous study. In this work, we performed the gain of function assay and find that overexpression of SHARPIN in LNCaP, DU145 and PC-3 cells promoted cell proliferation, invasiveness and reduced apoptosis. Furthermore, SHARPIN overexpression displayed elevated Bcl-2 and Survivin expression and reduced levels of Bax, cleaved caspase-3. Meanwhile, entropic expression of SHARPIN increased the levels of phosphorylated p65, IkB alpha, ERK and Akt, were selectively increased in these cells. Collectively, our study unraveled the ability of SHARPIN overexpression to induce tumorigenesis of prostate cancer cells through the NF-kB/ERK/Akt pathway and transformation of apoptosis-associated proteins.
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