Transcriptome of a specialized extra-embryonic cell, teratocyte, and its host immunosuppressive role revealed by ex vivo RNA interference

The specialized wasp cells teratocytes (TCs) are derived from the embryonic serosal membrane of some parasitic hymenopteran insects. As a parasitic factor, TCs are multifunctional in host regulation, such as host nutritional deprivation, immunosuppression and developmental arrest; however, little is understood about their genetic constituents. The present study provides a comprehensive view of the genes expressed by TCs through a transcriptome analysis based on RNA sequencing technology. The assembled 34686 contigs (>200 base pairs) were annotated into different functional categories, indicating a distinct distribution in gene transcripts compared with those of haemocytes and fat body. The TC transcriptome contained components of insulin signalling and biosyntheses of juvenile hormone and 20-hydroxyecdysone. TCs also expressed various groups of digestive enzymes, indicating that they have nutritional role for the growing parasitoid larvae in parasitism. Furthermore, through this transcriptome analysis two kinds of immunosuppressive serine protease inhibitors (serpins) and Rho GTPase-activating proteins (RhoGAPs) were annotated. To determine the biological functions of these factors, we devised ex vivoRNA interference (RNAi) by conducting knockdown of gene expression in in vitro-cultured TCs followed by injection of the treated TCs to test insects. Ex vivoRNAi revealed that some serpins and RhoGAPs expressed in TCs inhibited host cellular immunity. This study reports a transcriptome of the unique TC animal cell and its immunosuppressive genetic factors using ex vivoRNAi technology.