Journal
INSECT MOLECULAR BIOLOGY
Volume 22, Issue 5, Pages 485-496Publisher
WILEY
DOI: 10.1111/imb.12038
Keywords
Bemisia tabaci; metabolic resistance; thiamethoxam; microarray; stage specific
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Funding
- National Science Fund for Distinguished Young Scholars [31025020]
- National Basic Research Program of China [2013CB127602]
- Beijing Natural Science Foundation [6131002]
- 863 program [2012AA101502]
- National Technology Support Program [2012BAD19B06]
- Beijing Key Laboratory for Pest Control and Sustainable Cultivation of Vegetables, China
- BBSRC [BBS/E/C/00005193] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BBS/E/C/00005193] Funding Source: researchfish
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Bemisia tabaci has developed high levels of resistance to many insecticides including the neonicotinoids and there is strong evidence that for some compounds resistance is stage-specific. To investigate the molecular basis of B.tabaci resistance to the neonicotinoid thiamethoxam we used a custom whitefly microarray to compare gene expression in the egg, nymph and adult stages of a thiamethoxam-resistant strain (TH-R) with a susceptible strain (TH-S). Gene ontology and bioinformatic analyses revealed that in all life stages many of the differentially expressed transcripts encoded enzymes involved in metabolic processes and/or metabolism of xenobiotics. Several of these are candidate resistance genes and include the cytochrome P450 CYP6CM1, which has been shown to confer resistance to several neonicotinoids previously, a P450 belonging to the Cytochrome P450s 4 family and a glutathione S-transferase (GST) belonging to the sigma class. Finally several ATP-binding cassette transporters of the ABCG subfamily were highly over-expressed in the adult stage of the TH-R strain and may play a role in resistance by active efflux. Here, we evaluated both common and stage-specific gene expression signatures and identified several candidate resistance genes that may underlie B.tabaci resistance to thiamethoxam.
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