Journal
INSECT MOLECULAR BIOLOGY
Volume 20, Issue 3, Pages 409-415Publisher
WILEY
DOI: 10.1111/j.1365-2583.2011.01075.x
Keywords
circadian clock; cuticle deposition rhythm; mammalian-type cryptochrome; Riptortus pedestris; RNA interference
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Funding
- Japan Society for the Promotion of Science
- Institute of Low Temperature Science, Hokkaido University [08-20, 09-22]
- Grants-in-Aid for Scientific Research [22580057, 20380038] Funding Source: KAKEN
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Mammalian-type CRYPTOCHROME (CRY-m) is considered to be a core repressive component of the circadian clock in various insect species. However, this role is based only on the molecular function of CRY-m in cultured cells and it therefore remains unknown whether CRY-m is indispensable for governing physiological rhythms at the organismal level. In the present study, we show that RNA interference (RNAi) targeting of cry-m in the bean bug Riptortus pedestris disrupts the circadian clock governing the cuticle deposition rhythm and results in the generation of a single cuticle layer. Furthermore, period expression was induced in cry-m RNAi insects. These results verified that CRY-m functions as a negative regulator in the circadian clock that generates physiological rhythm at the organismal level.
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