Journal
INSECT MOLECULAR BIOLOGY
Volume 20, Issue 1, Pages 15-27Publisher
WILEY
DOI: 10.1111/j.1365-2583.2010.01046.x
Keywords
transposons; repetitive element; PCR competition
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Funding
- United States Department of Agriculture (USDA) Agriculture Research Service [3543]
- Iowa Agriculture and Home Economics Experiment Station, Ames [3543]
- USDA, Agricultural Research Service (ARS)
- CRIS [016]
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The movement of miniature inverted repeat transposable elements (MITEs) modifies genome structure and function. We describe the microsatellite-associated interspersed nuclear element 2 (MINE-2), that integrates at consensus WTTTT target sites, creates dinucleotide TT target site duplications (TSDs), and forms predicted MITE-like secondary structures; a 5' subterminal inverted repeat (SIR; AGGGTTCCGTAG) that is partially complementary to a 5' inverted repeat (IR; ACGAAGCCCT) and 3'-SIRs (TTACGGAACCCT). A (GTCY)(n) microsatellite is hitchhiking downstream of conserved 5' MINE-2 secondary structures, causing flanking sequence similarity amongst mobile microsatellite loci. Transfection of insect cell lines indicates that MITE-like secondary structures are sufficient to mediate genome integration, and provides insight into the transposition mechanism used by MINE-2s.
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