Journal
INSECT MOLECULAR BIOLOGY
Volume 19, Issue 6, Pages 753-763Publisher
WILEY
DOI: 10.1111/j.1365-2583.2010.01032.x
Keywords
dengue; mosquito; salivary glands; promoter; transgenesis; Aedes aegypti
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Funding
- Foundation for the National Institutes of Health through the Grand Challenges in Global Health Initiative
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Controlled sex-, stage- and tissue-specific expression of antipathogen effector molecules is important for genetic engineering strategies to control mosquito-borne diseases. Adult female salivary glands are involved in pathogen transmission to human hosts and are target sites for expression of antipathogen effector molecules. The Aedes aegypti 30K a and 30K b genes are expressed exclusively in adult female salivary glands and are transcribed divergently from start sites separated by 263 nucleotides. The intergenic, 5'- and 3'-end untranslated regions of both genes are sufficient to express simultaneously two different transgene products in the distal-lateral lobes of the female salivary glands. An antidengue effector gene, membranes no protein (Mnp), driven by the 30K b promoter, expresses an inverted-repeat RNA with sequences derived from the premembrane protein-encoding region of the dengue virus serotype 2 genome and reduces significantly the prevalence and mean intensities of viral infection in mosquito salivary glands and saliva.
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