Journal
INSECT MOLECULAR BIOLOGY
Volume 17, Issue 6, Pages 597-606Publisher
BLACKWELL PUBLISHING
DOI: 10.1111/j.1365-2583.2008.00831.x
Keywords
Rhipicephalus (Boophilus) microplus; acaricide resistance; organophosphates; microarrays; detoxification enzymes
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Funding
- National Research Initiative of the USDA CSREES [2005-35604-15440]
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Acaricide-inducible differential gene expression was studied in larvae of Rhipicephalus (Boophilus) microplus using a microarray-based approach. The acaricides used were: coumaphos, permethrin, ivermectin, and amitraz. The microarrays contained over 13 000 probes, having been derived from a previously described R. microplus gene index (BmiGI Version 2; Wang et al., 2007). Relative quantitative reverse transcriptase-PCR, real time PCR, and serial analysis of gene expression data was used to verify microarray data. Among the differentially expressed genes with informative annotation were legumain, glutathione S-transferase, and a putative salivary gland-associated protein.
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