Journal
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
Volume 49, Issue -, Pages 35-42Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.ibmb.2014.03.010
Keywords
CRISPR/Cas9; Bombyx mod; Mutagenesis; Chromosomal deletion; Chromosomal inversion; Multiplex genome editing
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Funding
- National Basic Research Program of China (973 program) [2012CB114600]
- National High-tech R&D Program (863 program) [2011AA100306]
- doctoral Innovation Fund of Southwest University [kb2010002]
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Bombyx mari is an economically important insect and a model organism for studying lepidopteran and arthropod biology. Using a highly efficient CRISPR/Cas9 system, we showed that this system could mediate highly efficient targeted genome editing of a single gene locus, large chromosomal deletion or inversion, and also multiplex genome editing of 6 genes simultaneously in BmNs cell line derived from B. mori. The simplicity and high efficiency of our system provide unprecedented possibilities for researchers to implement precise and sophisticated manipulation of a chosen B. mori gene in BmNs cells easily in a limited time course, and perhaps new opportunities for functional genomics of B. mori and other lepidopteran insects. (C) 2014 Elsevier Ltd. All rights reserved.
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