Journal
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
Volume 38, Issue 5, Pages 552-567Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.ibmb.2008.01.005
Keywords
moth sex-pheromones; regulation; PBAN; biosynthesis; ACCase; Plodia interpunctella; Helicoverpa armigera
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We combine the use of labeled precursors with enzyme inhibitors to decipher the biosynthetic pathway of pheromone biosynthesis and the rate-limiting step/s that are regulated by pheromone biosynthesis activating neuropeptide (PBAN). We demonstrate that Plodia interpunctella is able to utilize hexadecanoic acid, and to a lesser extent tetradecanoic acid, for the biosynthesis of the main pheromone component (Z, E)- 9,12-tetradecadienylacetate. This indicated that the main pathway involves a Delta 11 desaturase, chain shortening, followed by a Delta 12 desaturase, but that a functional Delta 9 desaturase could also be utilized. Using reverse transcription-quantitative real-time polymerase chain reaction (RT-QPCR) we distinguish two out of nine possible desaturase gene transcripts in P. interpunctella that are expressed at the highest levels. The rate-limiting step for PBAN-stimulation was studied in two moth species so as to compare the biosynthesis of a diene (P. interpunctella) and a monoene (Helicoverpa armigera) main pheromone component. In both species, incorporation of label from the C-13 sodium acetate precursor was activated by PBAN whereas no stimulatory action was observed in the incorporation of the precursors: C-13 malonyl coenzyme A; hexadecanoic 16,16,16-H-2(3) or tetradecanoic 14,14,14-H-2(3) acids. The acetyl coenzyme A carboxylase (ACCase) inhibitor, Tralkoxydim, inhibited the PBAN-stimulation of incorporation of stable isotope whereas the fatty-acyl reductase inhibitor, Mevastatin.. failed to influence the stimulatory action of PBAN. These results provide irrefutable support to the hypothesis that PBAN affects the production of malonyl coenzyme A from acetate by the action of ACCase in the pheromone glands of these moths. (C) 2008 Elsevier Ltd. All rights reserved.
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