Tumor accumulation of neutron-activatable holmium-containing mesoporous silica nanoparticles in an orthotopic non-small cell lung cancer mouse model

Mesoporous silica MCM-41 nanoparticles containing the stable isotope holmium-165 (Ho-165) were prepared by exposing the approximately 400 nm particles to an aqueous solution of Ho-165 acetylacetonate for 24 h at room temperature; the obtained solid was subsequently irradiated in a PULSTAR nuclear reactor (reactor power = 1 MW; thermal neutron flux of approximately 5.5 or 7.7 x 10(12) n/cm(2)s) to produce holmium-166-containing mesoporous silica (Ho-166-MCM-41) nanoparticles (20.8 +/- 1.9% w/w Ho-166). The Ho-166-MCM-41 nanoparticles were administered intravenously (i.v.) to orthotopic non-small cell lung cancer A549-luciferase tumor-bearing mice. After 24 h, 4.5 +/- 3.9% initial dose per gram (ID/g) of tissue was detected in tumors and after 1 week, this value increased to 58.8 +/- 34.7% ID/g. These results demonstrate that mesoporous silica MCM-41 nanoparticles can deliver significant amounts of a therapeutic radionuclide to tumors after i.v. injection. (C) 2012 Elsevier B.V. All rights reserved.