Journal
INORGANICA CHIMICA ACTA
Volume 361, Issue 14-15, Pages 4129-4137Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.ica.2008.03.091
Keywords
Trametes trogii; Funalia trogii laccase; X-ray structure
Categories
Funding
- Progetto MECHOS [POR Ob. 3 2000/2006 Toscana]
- Progetti integrati di ricerca Mis D4 [1785]
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A multicopper oxidase, the fungal laccase glycoenzyme from the white-rot basidiomycete fungus Trametes (Funalia) trogii, was crystallized and its crystal structure was solved at 1.58 angstrom using molecular replacement techniques. Model refinement resulted in R-factor and R-free values of 17.4% and 19.0%, respectively. The T. trogii laccase structural model reveals the presence of a ligand bound to the T1 active site which resembles a p-toluate molecule, such bound compound is most probably a fungal metabolite. The p-toluate is bound into the T1 active site of the laccase forming, with one of the carboxylate oxygens, a H-bond with His455, one of the T1 copper ion ligands, whereas the methyl group presents hydrophobic interactions within a pocket composed by Phe331, Phe336, Pro390 and Val162. The coordination geometries, the bond distances and the oxidation states of the T1 and T2/T3 copper active sites are analyzed and discussed in terms of the enzymatic mechanism and catalytic functionality. (C) 2008 Elsevier B. V. All rights reserved.
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