Journal
INORGANIC CHEMISTRY
Volume 52, Issue 17, Pages 10131-10136Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ic401531r
Keywords
-
Categories
Funding
- NIH [GM33309]
- Lindemann Trust
- Tobacco-Related Disease Research Program (TRDRP)
Ask authors/readers for more resources
The luminescence of rac-[Ru(bpy)(2)(dppz)](2+) (bpy = 2,2'bipyridine and dppz = dipyrido[3,2-a:2',3'-c]phenazine) was explored in the presence of RNA oligonucleotides containing a single RNA mismatch (CA and GG) in order to develop a probe for RNA mismatches. While there is minimal luminescence of [Ru(bpy)(2)(dppz)](2+) in the presence of matched RNA due to weak binding, the luminescence is significantly enhanced in the presence of a single CA mismatch. The luminescence differential between CA mismatched and matched RNA is substantially higher compared to the DNA analogue, and therefore, [Ru(bpy)(2)(dppz)](2+) appears to be also a sensitive light switch probe ' for a CA mismatch in duplex RNA. Although the luminescence intensity is lower in the presence of RNA than DNA, Forster resonance energy.transfer (FRET) between the donor ruthenium 'complex and 'FRET acceptor SYTO 61 is successfully exploited to amplify the luminescence in the presence of the mismatch. Luminescence and quenching studies with sodium iodide suggest that [Ru(bpy)(2)(dppz)](2+) binds to these mismatches via rnetalloinsertion from the minor groove. This work provides further evidence that metalloinsertion is a general binding mode. of octahedral metal complexes to thermodynamically destabilized mismatches not only in DNA but also in RNA.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available