[OsIVCl5(Hazole)]- Complexes: Synthesis, Structure, Spectroscopic Properties, and Anti proliferative Activity

By exploring the Anderson type rearrangement reactions, osmium(IV) complexes of the general formula [cation](+) [(OsCl5)-Cl-IV(Hazole)](-), where [cation](+) = n-Bu4N+, Hazole = 1H-pyrazole (Hpz) (1), 1H-indazole (Hind) (2), 1H-imidazole (Him) (3), 1H-benzimidazole (Hbzim) (4), 1H,2,4-triazole (Htrz) (5), have been synthesized. To improve water solubility of tetrabutylammonium compounds, complexes with [cation](+) = Na+ (Hazole = Hpz 6), Hind (7), Htrz (8)] or H(2)azole(+) [Hazole = Hpz (9), Hind (10), Htrz (11)] have been also prepared with the aim of testing them for cytotoxicity in cancer cells. In addition, the preparation of the complex {(n-Bu4N)(2)[(OSCl6)-Cl-IV]}(2)[(OsCl4)-Cl-IV(Him)(2)] (12) is also reported. The compounds have been comprehensively characterized by elemental analysis, electrospray ionization (ESI) mass spectrometry, spectroscopy (IR, UV-vis, 1D and 2D NMR), cyclic voltammetry, X-ray crystallography (1-6 and 12) and magnetic susceptibility (5). Complexes 6, 7, 9 are kinetically inert in aqueous solution and resistant to hydrolysis. Compounds 6-11 were found to possess modest antiproliferative acitivity in vitro against CH1 (ovarian carcinoma), A549 (non-small cell lung carcinoma), and SW480 (colon adenocarcinoma) cells with IC50 values in the 10(-4) M concentration range. Replacement of azolium cations by sodium had significant effects; cytotoxicity increased in the case of the pyrazole system from 3 (A549) to the 5.5-fold (CH1).