Journal
INNATE IMMUNITY
Volume 18, Issue 6, Pages 856-865Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1177/1753425912444479
Keywords
TLR4; RSV; innate immunity
Categories
Funding
- Canadian Institutes of Health Research Team in Mutagenesis and Infectious Diseases
- AllerGen's Canadian Allergy and Immune Diseases Advanced Training Initiative
- Canadian Institutes of Health Research
- Canadian Lung Association
- GlaxoSmithKline Inc.
Ask authors/readers for more resources
TLRs play a key role in innate immune defenses. It was previously reported that purified respiratory syncytial virus (RSV) fusion protein elicits an inflammatory response in hematopoietic cells, which required expression of TLR4 and its co-receptor CD14. However, a biological role of TLR4 in immunity to RSV, as initially proposed, has remained inconclusive and controversial. Here, we directly assess the role of human TLR4 and its co-receptors in NF-kappa B activation, viral entry and replication using intact virions rather than purified RSV components. We used HEK 293 reporter cells that are highly permissive for RSV and that either express or a lack a functional human TLR4/MD-2/CD14 complex. We demonstrate that RSV-mediated NF-kappa B activation, viral entry and replication are independent of the expression of a functional human TLR4/MD-2/CD14 complex and that, in turn, human TLR4 activation by LPS remains unaffected in RSV-infected cells. Thus, although isolated viral compounds such as purified RSV F protein may bind TLR4 and/or CD14, a direct interaction between intact RSV particles and the human TLR4 receptor complex does not seem to play a biological role in RSV pathogenesis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available