Journal
MECHANISMS OF AGEING AND DEVELOPMENT
Volume 150, Issue -, Pages 55-64Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mad.2015.08.005
Keywords
Anandamide; 2-Arachidonoylglycerol; Monoacylglycerol lipase; sn-1-diacylglycerol lipase; LC-MRM
Categories
Funding
- German Research Foundation [FOR926, SP2, SP3, CP1, BI 1227/5-1]
Ask authors/readers for more resources
Previous studies have demonstrated that the endocannabinoid system significantly influences the progression of brain ageing, and the hippocampus is one of the brain regions most vulnerable to ageing and neurodegeneration. We have further examined age-related changes in the hippocampal endocannabinoid system by measuring the levels of anandamide (AEA) and 2-arachidonoylglycerol (2-AG) in young and old mice from two different mouse strains. We found a decrease in 2-AG but not AEA levels in aged mice. In order to identify the cause for 2-AG level changes, we investigated the levels of several enzymes that contribute to synthesis and degradation of 2-AG in the hippocampus. We found a selective decrease in DAGL alpha mRNA and protein levels as well as an elevated MAGL activity during ageing. We hypothesize that the observed decrease of 2-AG levels is probably caused by changes in DAGL alpha expression and MAGL activity. This finding can contribute to the existing knowledge about the processes underlying selective vulnerability of the hippocampus to ageing and age-related neurodegeneration. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available