Journal
INFLAMMATORY BOWEL DISEASES
Volume 14, Issue 8, Pages 1041-1050Publisher
JOHN WILEY & SONS INC
DOI: 10.1002/ibd.20442
Keywords
IL-10(-/-) mice; rRNA genes; Crohn's disease; IBD; bacteria
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Funding
- NIAID NIH HHS [R01 AI078885] Funding Source: Medline
- NIDDK NIH HHS [P01 DK046763] Funding Source: Medline
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Background: Microorganisms appear to play important yet ill-defined roles in the etiology of infammatory bowel disease (IBD). This study utilised a novel population-based approach to identify bacteria and bacterial rRNA genes associated with the development of colitis in IL-10(-/-) mice. Methods: Mice were housed in 2 environments: a community mouse facility where the; mice were fed nonsterile chow (Room 3) and a limited access facility where the mice were fed sterile chow (Room 4). Every month the disease activity levels were assessed and fecal bacterial compositions were analyzed. At the end of the experiments histological and bacterial analyses were performed on intestinal tissue. Results: Although disease activity increased over time in both environments, it progressed at a faster rate in Room 3 than Room 4. Culture and culture-independent bacterial analyses identified several isolates and phylotypes associated with colitis. Two phylotypes (GpC2 and Gp66) were distinguished by their negative associations with disease activity in fecal and tissue samples. Notably, rRNA genes from these phylotypes had high sequence identity (99%) to an rRNA gene from a previously described flagellated Clostridium (Lachnospiraceae bacterium A4). Conclusions: The negative associations of these 2 phylotypes (GpC2 and Gp66) suggest that these bacteria were being immunologically targeted, consistent with prior findings that the Lachnospiraceae bacterium A4 bears a prevalent flagellar antigen for disease-associated immunity in marine immune colitis and human Crohn's disease. Identification of these associations suggests that the experimental approach used in this study will have considerable utility in elucidating the host-microbe interactions underlying IBD.
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