Journal
INFLAMMATION RESEARCH
Volume 58, Issue 10, Pages 705-715Publisher
SPRINGER BASEL AG
DOI: 10.1007/s00011-009-0039-1
Keywords
Mast cells; Heme oxygenase-1; Flavonoids; Degranulation; Quercetin
Categories
Funding
- Japan Society for the Promotion of Science
- Nagono Medical Foundation, Nagoya, Japan
Ask authors/readers for more resources
We investigated the involvement of heme oxygenase (HO)-1 in the anti-allergic action of quercetin against degranulation of rat basophilic leukemia (RBL-2H3) cells, rat peritoneal mast cells, and mouse bone marrow-derived mast cells. The strength of allergic reaction was evaluated by the extent of degranulation in mast cells sensitized with various stimulants. The levels of HO-1, HO-2, and nuclear factor erythroid 2-related factor 2 (Nrf2) expressions were determined by quantitative RT-PCR, western blotting, or immunocytochemistry. Heme oxygenase activity was upregulated after short exposure to quercetin, followed by the induction of HO-1 expression after long exposure to quercetin. The inhibition of degranulation by quercetin was reversed using tin protoporphyrin IX (SnPP), an HO-1 inhibitor. HO-1 metabolites, bilirubin and CO, led to inhibit degranulation, and quercetin translocated Nrf2 from cytoplasm into nucleus in RBL-2H3 cells. These results strongly suggest that quercetin exerted anti-allergic actions via activation of Nrf2-HO-1 pathway.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available