Journal
INFLAMMATION
Volume 42, Issue 1, Pages 331-341Publisher
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10753-018-0896-2
Keywords
rheumatoid arthritis; miR-410-3p; fibroblast-like synoviocytes; cytokines; NF-kappa B signaling
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Funding
- program of the foundation in Shenyang [D267, MF55]
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miR-410-3p acts as an oncogene or a tumor suppressor in some malignancies. However, its role in rheumatoid arthritis (RA) is unknown. The study was conducted to investigate the effect of miR-410-3p on the pathogenesis of RA. Real-time RT-PCR was used to determine the mRNA levels of miR-410-3p in synovial tissues and fibroblast-like synoviocytes (FLSs). An ELISA was performed to examine the production levels of tumor necrosis factor (TNF)-, interleukin (IL)-1, IL-6, and matrix metalloproteinase (MMP)-9. Western blotting was conducted to determine the protein levels of IB-, p-IB, p65, and p-p65. Nuclear factor (NF)-B activation and nuclear translocation assays were performed to confirm the activation of NF-B. We found that the expression level of miR-410-3p was downregulated in synovial tissues and FLSs from RA. Overexpression of miR-410-3p significantly reduced the secretion of TNF-, IL-1, IL-6, and MMP-9 in human RA fibroblast-like synoviocytes (HFLS-RA); whereas miR-410-3p inhibition increased the expression levels of these cytokines. Furthermore, miR-410-3p suppresses the activation of NF-B signaling pathway. Moreover, NF-B inhibitor restored the elevation of TNF-, IL-1, IL-6, and MMP-9 induced by miR-410-3p inhibition. Our results demonstrate that miR-410-3p acts an inflammatory suppressor in the pathogenesis of RA by regulating the NF-B signaling pathway. These data suggest a novel function of miR-410-3p and provide insight into the complex mechanisms involved in RA.
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