Journal
INFLAMMATION
Volume 38, Issue 3, Pages 978-986Publisher
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10753-014-0060-6
Keywords
periodontitis; interleukin-17F (IL-17F); interleukin-17A (IL-17A)
Categories
Funding
- National Natural Science Foundation of China [81371150, 81400517]
- China Postdoctoral Science Foundation [2014M550767]
- Beijing Postdoctoral Research Foundation [2014ZZ-02]
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The objective of this study was to investigate the expression level, clinical significance, and possible regulating role of IL-17F in patients of chronic periodontitis. Periodontal local tissues were obtained from chronic periodontitis (CP) and healthy controls (HC) for real-time PCR (RT-PCR) detection with IL-17F and IL-17A messenger RNA (mRNA). Primary human gingival fibroblasts (HGF) were derived from patients receiving crown-lengthening procedures. Efficiency of small interfering RNA (siRNA) of IL-17R to HGF cells were assessed by Western blot and RT-PCR. Recombinant IL-17F and IL-17A were used to stimulate the HGF cells compared with the control group. Aspects of the nuclear factor-kappa B (NF-kappa B) and extracellular signal-regulated kinase (ERK) signaling pathways were examined by Western blot. Production of pro-inflammatory cytokines induced by IL-17F and IL-17A was detected by RT-PCR. Statistical analysis was analyzed by SPSS software. It showed significantly elevated levels of IL-17F and IL-17A mRNA in CP gingival tissues compared with HC group (P< 0.01). Further analysis showed a significant correlation between IL-17F and IL-17A mRNA in CP group (P< 0.05), and both cytokines also correlated with the probing depth (P< 0.05). Recombinant IL-17F can induce NF-kappa B phosphor-p65 and ERK phosphorylation of HGF cells similar to that of IL-17A. Interestingly, we found that both IL-17F and IL-17A could promote the important inflammatory cytokines IL-6, CXCL8, and CCL20 production compared with IL-17R siRNA group (P< 0.05). This study indicates that IL-17F may be involved in pathogenesis of periodontitis like IL-17A. The role of IL-17F in disease pathogenesis needs to be further investigated.
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