Journal
INFLAMMATION
Volume 36, Issue 6, Pages 1201-1208Publisher
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10753-013-9656-5
Keywords
unfractionated heparin; lipopolysaccharide; endotoxemia; acute lung injury; nuclear factor-kappa B
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Funding
- National Natural Science Foundation of China [81101411]
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The present study aimed to determine the protective effects and the underlying mechanisms of unfractionated heparin on lipopolysaccharide (LPS)-induced endotoxemia and lung injury in rats. Rats were injected intravenously with LPS at 6 mg/kg. We examined the therapeutic effects of unfractionated heparin (100 or 300 U/kg) on LPS-induced endotoxemia by dosing intravenously simultaneously after LPS challenge. The animal lung edema degree was evaluated by wet/dry weight ratio. The levels of inflammatory mediators including interleukin-1 beta (IL-1 beta) and interleukin-6 (IL-6) were assayed by enzyme-linked immunosorbent assay and quantitative real-time RT-PCR. The activation of nuclear factor-kappa B (NF-kappa B) was evaluated by Western blotting. The investigations revealed that treatment with unfractionated heparin can attenuate inflammatory responses in a rat model of LPS-induced acute lung injury, and the effect was much better in 300 U/kg group. The mechanisms by which unfractionated heparin exerts its anti-inflammatory effect are correlated with inhibition of IL-1 beta and IL-6 production via inactivation of NF-kappa B.
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